Polo-like kinase 1 (PLK1) is a serine/threonine protein kinase that plays a crucial role in regulating cell cycle progression, particularly during mitosis. It is involved in various mitotic events, including centrosome maturation, bipolar spindle formation, chromosome segregation, and cytokinesis. Dysregulation and overexpression of PLK1 are frequently observed in numerous human cancers, making it a significant therapeutic target in oncology for developing novel anti-cancer agents.