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Product name: Fructo-oligosaccharide DP9 / GF8
Synonym name:
Catalogue No.: BP4066
Cas No.: 143625-74-5
Formula: C54H92O46
Mol Weight: 1477.28
Botanical Source:
Physical Description: White powder
Type of Compound: Oligose
Purity: 95%~99%
Analysis Method: HPLC-DAD or/and HPLC-ELSD
Identification Method: Mass, NMR
Packing: Brown vial or HDPE plastic bottle
Storage: Store in a well closed container, protected from air and light. Put into refrigerate or freeze for long term storage.
Whenever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20℃. Generally, these will be useable for up to two weeks.
The product could be supplied from milligrams to grams
Inquire for bulk scale.
For Reference Standard and R&D, Not for Human Use Directly.
HPLC of Fructo-oligosaccharide DP9 / GF8
HNMR of Fructo-oligosaccharide DP9 / GF8
Storage conditions：Short-term storage at 2~8℃, long-term storage at -20 ~ -80℃
743.5800
-5.2334
-5.2334
29.4700
.4314
.1689
Low
18.2355
7.7969
Yes
No
No
No
Yes
No
0.9
Yes
Yes
Yes
Yes
Fructo-oligosaccharide (DP9, abbreviated as FOS DP9, CAS No. 143625-74-5) is a fructo-oligosaccharide composed of nine fructose units connected by β-(2→1) glycosidic bonds, belonging to the middle and long chains of the fructo-oligosaccharides (FOS) family. As a typical prebiotic, sucrose nectarin has attracted widespread attention in recent years in the fields of food, nutrition, and medicine due to its unique physiological functions and good safety. Its main mechanisms of action include regulating intestinal microecological balance, enhancing intestinal barrier function, modulating immune responses, and anti-inflammatory effects, involving multiple molecular targets such as TLR4, TLR2, MUC2, IL22, OCLN, ZO1, GPR43, GPR41, CLDN1, and the Bifidobacterium genus (BIFIDO).
This review aims to systematically summarize the chemical structure and physicochemical properties of sucrose nine-sugar cane sugarcane, plant origin and extraction methods, pharmacological activity and mechanism, druggability evaluation, and pharmacokinetic characteristics. Combined with current clinical application research, it explores its potential value and future development directions in the prevention and treatment of gut-related diseases.
Sucra nine-sugar is a fructooligosaccharide composed of nine fructose units, with a molecular formula of C54H90O45 and a molecular weight of approximately 1477.2840 Da. Its structural feature is a linear β-(2→1) fructose chain, with a glucose unit (GF8 structure) typically attached at the end, making it highly water-soluble and low-lipid. The LogP value was -5.2334, indicating strong polarity and difficulty penetrating lipid membranes, consistent with its physiological positioning as a gut prebiotic. The extremely large topological pole surface area (TPSA 743.58 Ų) further demonstrates its hydrophilicity and low cell membrane permeability.
The physicochemical properties of sucrose nine-sugar determine its stability and bioavailability in the gastrointestinal tract. Its high water solubility (29.47 mg/mL) ensures good solubility in the intestines, which is beneficial for gut microbes to utilize as a prebiotic. Its low blood-brain barrier permeability and lack of hERG channel inhibition demonstrate good safety and low neurotoxicity risk. Ames test results (0.9) showed no significant mutagenicity.
Nine sugarcane is mainly found in various plants, especially in the rhizomes of plants rich in fructooligosaccharides such as sugarcane (Saccharum officinarum) and chicory (Cichorium intybus). Sugarcane juice and chicory root extract are the main raw materials for industrial production of sucrose nine-sugar syrup. Although the content of natural fructooligosaccharides is limited, large-scale preparation of high-purity sucrose nine-saccharide can be achieved through enzymatic synthesis and biological fermentation technology.
Traditional extraction methods include water extraction, alcohol precipitation, and membrane separation techniques. Modern processes often use fructosyltransferase to catalyze sucrose conversion into fruit oligosaccharides, followed by ultrafiltration, nanofiltration, and chromatography purification to obtain high-purity sucrose heptose. This method is highly efficient, produces uniform products, and is suitable for industrial production.
As a prebiotic, sucrose nine-sugar mainly promotes the growth of beneficial gut bacteria (such as bifidobacteria and lactic acid bacteria), regulates the intestinal microecology, and exerts multiple pharmacological activities.
Numerous in vitro and animal studies have shown that sucrose nectaris significantly promotes the proliferation of bifidobacterium and lactic acid bacteria, inhibits the growth of pathogenic bacteria, and maintains gut microbiota balance. It promotes the production of probiotic metabolites such as short-chain fatty acids (SCFAs), improves the intestinal environment, and enhances gut health.
Sucralose enhances the integrity of the intestinal barrier by regulating the expression of tight junction proteins (such as OCLN, ZO1, CLDN1) in intestinal epithelial cells, preventing the invasion of harmful substances and pathogens, and reducing intestinal inflammation.
Sucralose can activate the intestinal immune system, regulate the TLR2 and TLR4 signaling pathways, promote the secretion of the anti-inflammatory cytokine IL-22, and regulate intestinal immune homeostasis, possessing potential anti-inflammatory and immunomodulatory effects.
By activating GPR43 and GPR41 receptors, sucrose promotes short-chain fatty acid-mediated metabolic signaling, regulates energy metabolism, and improves metabolic syndrome-related indicators.
The biological effects of sucrose nectar-sugar are mainly realized through gut microbial metabolism and host molecular target interactions.
As pattern recognition receptors, TLR2 and TLR4 play a key role in gut immunity. Sucra nectacan affects the downstream NF-κB signaling pathway by regulating the expression and activity of these two receptors, regulating inflammatory responses, and maintaining immune balance.
MUC2 is a major component of the intestinal mucus layer, protecting the intestinal epithelium from pathogen invasion. Sucra nectarate promotes MUC2 expression and enhances mucous barrier function.
These proteins maintain tight connections between intestinal epithelial cells, preventing increased intestinal permeability. Sucrose nine-sugar repairs the damaged intestinal barrier by upregulating the expression of these proteins.
As receptors for short-chain fatty acids, GPR41 and GPR43 mediate the signaling of sucrose nectar-sugar metabolites, regulating immune responses and energy metabolism.
Sucra nut nectaris selectively promotes the growth of Bifidobacterium, enhances its metabolic activity, produces beneficial metabolic products, and indirectly affects host health.
Druggability evaluation of sucrose nine-sugar shows good safety and biocompatibility. Its high molecular weight and polarity mean that after oral administration, it is mainly confined to the gastrointestinal tract and is difficult for intestinal absorption into systemic circulation, which aligns with the pharmacokinetic characteristics of prebiotics.
No hERG channel suppression, Ames test negative, indicating no significant cardiotoxicity or mutagenic risk. Preclinical toxicology studies have shown no significant toxic side effects.
Sucra nine-sugar is difficult to be enzymatically digested and absorbed by gastrointestinal enzymes; it is mainly fermented and utilized by gut microbes in the colon, producing short-chain fatty acids and other metabolites that exert local and systemic effects.
Its metabolism mainly depends on gut microbes and is not directly metabolized by host enzymes. The unabsorbed portion is excreted in feces.
As a safe and effective prebiotic, Sucrose has demonstrated potential in various clinical studies to improve intestinal function, alleviate Irritable Bowel Syndrome (IBS), inflammatory bowel disease (IBD), and promote gut health in children. Moreover, its role in regulating immunity and metabolism offers new ideas for adjunctive treatment of metabolic syndrome, allergic diseases, and immune-related conditions.
Future research should focus on:
These studies will promote the widespread application of sucrose nine-sugar in functional foods and biomedicine.
As a typical prebiotic, sucrose nine-sugar demonstrates significant pharmacological activity in regulating intestinal microecology, enhancing the intestinal barrier, and regulating immunity and metabolism due to its unique chemical structure and excellent physicochemical properties. Its mechanism of action involves multiple key molecular pathways and targets, reflecting a complex host-microbial interaction network. Druggability evaluations show good safety and suitability for long-term oral use. With increasing attention to the importance of gut health, the application prospects for sucrose nine-sugar in clinical and functional food fields are broad. Future research will further reveal its potential therapeutic value and application models, promoting it as an important natural product for the prevention and treatment of intestinal diseases and related metabolic diseases.
Shenshuai
Wenjing
Hedandan
