Product name: Fraxin
Synonym name: Paviin
Catalogue No.: BP0599
Cas No.: 524-30-1
Formula: C₁₆H₁₈O₁₀
Mol Weight: 370.31
Botanical Source: Fraxinus stylosa Lingelsh.
Physical Description:
Type of Compound: Coumarins

Purity: 95%~99%
Analysis Method: HPLC-DAD or/and HPLC-ELSD
Identification Method: Mass, NMR
Packing: Brown vial or HDPE plastic bottle

Storage: Store in a well closed container, protected from air and light. Put into refrigerate or freeze for long term storage.
Whenever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20℃. Generally, these will be useable for up to two weeks.

The product could be supplied from milligrams to grams
Inquire for bulk scale.




Chengdu Biopurify Phytochemicals Ltd. is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，TCM), Natural Active Pharmaceutical Ingredients worldwide.
The main services:
A. Supply active ingredients and reference standards of TCM, from mgs to kgs scale
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR(contract research), CM(contract manufacture) and PD(process development) services from lab scale, pilot scale to commercial scale(GMP is also available)
E. TCM compounds library for drug screen,more than 3000 compounds available, many of them are rare and unique.

HPLC of Fraxin

Storage conditions：Short-term storage at 2~8℃, long-term storage at -20 ~ -80℃

Introduction/Overview

Fraxin (CAS No.: 524-30-1) is a natural compound isolated from the traditional Chinese medicinal herb Cortex Fraxini, belonging to the glycoside compounds of the ash tree lactone. As a natural product with multiple biological activities, Qinpidin has seen increasing research in antioxidant, anti-inflammatory, antibacterial, and anti-tumor metastasis fields in recent years, demonstrating its potential medicinal value and development prospects. This paper aims to systematically review the chemical structure, physicochemical properties, plant origin, extraction methods, pharmacological activity, and mechanism of action of Qin Pirin, and, combined with druggability evaluation and pharmacokinetic characteristics, explores its clinical application prospects and future research directions, providing theoretical basis and reference for natural product pharmacology and new drug development.

Chemical structure and physicochemical properties

Qin Peidin is a glycoside of the alkali lactone, with a molecular formula of C17H18O10 and a molecular weight of 370.3100. Its structural features include an lactone ring connected to the glucosinolate section, giving it high polarity. The LogP value of Qinperidin is -0.4061, indicating strong hydrophilicity, water solubility of 8.8525, good water solubility, and suitable for biological activity in aqueous phase systems. Its topological pole surface area (TPSA) is 159.05 Ų, suggesting that its molecules have a high number of polar groups, which may affect membrane permeability and bioavailability.

Additionally, Qinpeidin has a relatively low blood-brain barrier penetration capacity, suggesting its role in the central nervous system may be limited, but this also reduces its potential risk of CNS toxicity. The hERG channel inhibition test was negative, indicating that qinperidin is less likely to cause cardiac QT prolongation and is relatively safe. The Ames mutagenic test scored 0.9, indicating a low genotoxicity risk and meeting safety requirements for drug development.

Plant Origins and Extraction Methods

Fraxinin is mainly found in the bark of the oleaceae plant Fraxinus spp., with abundant content in Cortex fraxini. As a traditional Chinese medicinal herb, Qin Pi is widely used in traditional treatments such as clearing heat and drying dampness, purging fire, and detoxifying. Qinperidin is usually extracted using water extraction and alcohol precipitation or organic solvent extraction methods, combined with column chromatography, countercurrent chromatography, and other separation and purification techniques to obtain high-purity Qinperidin.

The typical extraction process generally includes: first, the dried Qin pi is crushed, then refluxed extraction with 70%-80% ethanol or methanol. After concentration, the extract is separated and purified using a silica gel column or a C18 reversed-phase column. In recent years, the application of ultrasound-assisted extraction and microwave-assisted extraction technologies has improved the extraction efficiency and purity of qin pei, while reducing solvent usage and extraction time, aligning with the concept of green chemistry.

Pharmacological activity research

Antioxidant activity

Qinperidin exhibits significant antioxidant effects, scavenging free radicals and reducing cellular damage caused by oxidative stress. Multiple in vitro experiments have shown that qin peidin can effectively scavenge DPPH free radicals, superoxide anions, and hydroxyl radicals, protecting cells from oxidative damage. Its antioxidant activity is closely related to its abundant hydroxyl and lactone rings in its structure, and it can neutralize free radicals through electron donors.

Anti-inflammatory activity

Qinperidin demonstrates good anti-inflammatory effects across various inflammatory models. In vitro cell experiments show that qin pedicin can inhibit the expression of pro-inflammatory factors such as TNF-α, IL-1β, and IL-6, thereby reducing inflammatory responses. In animal models, Qinpidin regulates the NF-κB signaling pathway, inhibits the release of inflammatory mediators, and significantly reduces tissue inflammatory damage.

Antibacterial activity

Qinperidin exhibits inhibitory effects on various bacteria and fungi. Its antibacterial targets involve key enzymes such as bacterial DNA gyrase (GYRA), cell wall synthase (FABI), and dihydrofolate reductase (DHFR), which can interfere with bacterial DNA replication and cell wall synthesis. Inhibition of the fungal target ERG11 (encoding cytochrome P450 14α-demethylase) and its homologous enzyme CYP51A1 imparts antifungal activity to qinperidin. Additionally, Qinpeidin can inhibit the fungal multidrug resistance protein CDR1, enhancing the efficacy of antifungal drugs.

Antimetastatic activity

Research on Qin Piridin in inhibiting tumor metastasis has gradually increased. In vitro experiments have shown that Qin Peridin can inhibit tumor cell migration and invasion, reduce the expression of matrix metalloproteinases (MMPs), block the interaction between tumor cells and the stroma, and thereby suppress tumor metastasis.

Mechanism of action and molecular targets

The multi-target mechanism of Qinpeidin is the foundation for its multiple pharmacological activities. Its antioxidant effects mainly work by inhibiting cyclic adenylate phosphodiesterase (PDE) activity, regulating intracellular cAMP levels, activating downstream antioxidant enzyme systems, and reducing the generation of reactive oxygen species (ROS). The anti-inflammatory mechanism involves inhibiting the NF-κB signaling pathway, blocking the transcription and release of pro-inflammatory factors.

In terms of antibacterial activity, Qin Pidin blocks DNA replication by binding to bacterial DNA gyra. Inhibits the cell wall synthase FABI, interfering with bacterial cell wall synthesis; At the same time, it inhibits DHFR, blocks nucleotide synthesis, and suppresses bacterial proliferation. For fungi, qinperidin targets ERG11 and CYP51A1, blocking ergosterol biosynthesis and damaging cell membrane integrity. Additionally, by inhibiting CDR1, qinperidin reduces the fungal's drug elimination ability and increases the sensitivity of antifungal drugs.

In terms of anti-metastatic effects, qin pidin downregulates MMPs and related signaling pathways, inhibiting matrix degradation and migration of tumor cells, thereby blocking the metastasis process.

Druggability evaluation and pharmacokinetics

The physicochemical properties of Qin Pidin show good water solubility, making it suitable for oral administration. Its LogP value and TPSA indicate that qinperidin is highly polar and has limited membrane permeability, especially low blood-brain barrier permeability, which may limit pharmacological effects in the central nervous system but also reduce the risk of central toxicity. The inhibition of the hERG channel and its low genotoxicity risk ensure its safety.

Currently, pharmacokinetic studies on Qinpeidin are limited. Preliminary data indicate that oral absorption is relatively slow, and its bioavailability is limited by its high polarity and glycoside structure hydrolysis stability. Metabolic pathways may involve enzymatic hydrolysis in the liver and corresponding glucosidase effects; the activity and toxicity of these metabolites require further study. The excretion route of qinperidin may mainly be excreted by the kidneys.

Prospects and outlooks for clinical applications

Qinpeidin, as a multifunctional natural product, possesses multiple pharmacological activities including antioxidant, anti-inflammatory, antibacterial, and anti-tumor metastasis, demonstrating broad clinical application potential. Its antioxidant and anti-inflammatory effects give it potential therapeutic value in chronic inflammatory diseases, cardiovascular diseases, and metabolic syndromes. Its antibacterial activity provides new drug candidates in the field of anti-infectivity, especially its promising application prospects against multidrug-resistant strains.

Additionally, Qinperidin's inhibitory effect on tumor metastasis provides a theoretical basis for its development as an adjunct antitumor drug. In the future, structural modification and drug carrier technologies can improve bioavailability and targeting to enhance clinical efficacy.

However, the clinical translation of Qinpidin still faces many challenges, including in vivo metabolic stability, unclear pharmacokinetic characteristics, and a lack of systematic toxicological evaluation and clinical trial data. Future research needs to strengthen pharmacokinetics and safety evaluation of Qin Pidin, conduct more mechanistic studies and animal model validation, and promote its advancement toward clinical application.

Conclusion

Qin Pi Ding, a type of glycoside derived from the traditional Chinese medicine Qin Pi, demonstrates a wide range of biological functions and good safety profiles due to its unique chemical structure and multi-target pharmacological activity. Its antioxidant, anti-inflammatory, antibacterial, and anti-tumor metastasis activities provide abundant research resources for new drug development. In the future, by integrating modern medicinal chemistry, molecular biology, and pharmacokinetic research, optimizing the properties and clinical application strategies of Qinperidin is expected to make it an important candidate molecule for natural product drug development, benefiting the clinical treatment field.