Product name: Timosaponin BII
Synonym name:
Catalogue No.: BP1396
Cas No.: 136656-07-0
Formula: C₄₅H₇₆O₁₉
Mol Weight: 921.084
Botanical Source: Anemarrhena asphodeloides Bge.
Physical Description:
Type of Compound: Steroids

Purity: 95%~99%
Analysis Method: HPLC-DAD or/and HPLC-ELSD
Identification Method: Mass, NMR
Packing: Brown vial or HDPE plastic bottle

Storage: Store in a well closed container, protected from air and light. Put into refrigerate or freeze for long term storage.

The product could be supplied from milligrams to grams
Inquire for bulk scale.

Description:

Timosaponin BII has anti-diabetes, antioxidant, anti-inflammatory, and anti-dementia activities, it can significantly reduce the neurotoxicity induced by beta amyloid peptide 25-35 in primary neurons, the mechanism of which may be related with resisting oxidative damage and regulating the cholinergic system.Timosaponin BII can improve the neurological symptoms of cerebral ischemic rat, reduce infarct size, relieve brain water edema, improve hemorheology, reduce inflammatory injury of cerebral ischemia; it could be used in the preparation of a medicament or product for the prevention and treatment of stroke.

References:

Chinese Pharmacological Bulletin, 2009, 25(2):244-7.    

Protective effects of timosaponin BII on primary neurons against beta amyloid peptide 25-35.    

To study the protective effects of Timosaponin BII on primary neurons against beta amyloid peptide 25-35 induced toxicity. 

METHODS AND RESULTS:

Immunofluorescence was used to identify primary neurons. MTT(tetrazolium salt) assay was used to measure neurons metabolic state. Spectrophotometric method was used to measure the release of LDH (lactate dehydrogenase), the activity of SOD (superoxide dismutase), the activity of AChE (acetylcholine) and the production of MDA(malonaldehyde) in the culture medium.Treatment with beta amyloid peptide 25-35 (20 μmol · L-1) for 24 h caused a significant damage in primary neurons. Timosaponin BII 10-4 10-5 mol · L-1 markedly improved neurons metabolic activity, decresed the release of LDH and the production of MDA, markedly increased the activity of SOD and decresed the activity of AChE. 

CONCLUSIONS:

Timosaponin BII could significantly reduce the neurotoxicity induced by beta amyloid peptide 25-35 in primary neurons. The mechanism of which may be related with resisting oxidative damage and regulating the cholinergic system.    

Planta Med. 2012 Jan;78(1):18-23. doi: 10.1055/s-0031-1280268.    

Anti-diabetic effects of TongGuanWan, a Chinese traditional herbal formula, in C57BL/KsJ-db/db mice.  

In the present study, the anti-diabetic effects of a traditional Chinese medicinal formula extract, TongGuanWan, were investigated in type 2 diabetic animals. 

METHODS AND RESULTS:

It was orally administered to C57BL/KsJ-db/db mice once a day for 4 weeks at the doses of 62, 125, and 250 mg/kg body weight. TongGuanWan significantly lowered the blood glucose and glycosylated haemoglobin levels as well as improved the glucose tolerance in db/db mice. The serum triglyceride levels in the db/db mice were significantly decreased, whereas the high-density lipoprotein cholesterol levels were significantly increased, after treatment with this herbal formula. TongGuanWan also markedly decreased the animals' body weights compared to those of the control db/db group but did not alter food intake. The effects of TongGuanWan were compared to those of the drug rosiglitazone. In addition, five main constituents of TongGuanWan, mangiferin, berberine, cinnamic aldehyde, Timosaponin BII, and timosaponin AIII, were quantified using high performance liquid chromatography coupled with a diode array and an evaporative light scattering detector (HPLC-DAD-ELSD).

CONCLUSIONS:

These results suggest that TongGuanWan may be useful for the treatment of type 2 diabetes.    

US20090075912[P]. 2009.    

Use of Timosaponin BII in the Preparation Of A Medicament or Product for the Prevention and Treatment of Stroke    

The invention disclosed the use of Timosaponin BII in the preparation of a medicament or product for the prevention and treatment of stroke. The experiments prove that Timosaponin BII can improve the neurological symptoms of cerebral ischemic rat, reduce infarct size, relieve brain water edema, improve hemorheology, reduce inflammatory injury of cerebral ischemia.    

HPLC of Timosaponin BII

HNMR of Timosaponin BII

Storage conditions：Short-term storage at 2~8℃, long-term storage at -20 ~ -80℃