Product name: Butin
Synonym name: 7, 3′, 4′-trihydroxydihydroflavone
Catalogue No.: BP0295
Cas No.: 492-14-8
Formula: C₁₅H₁₂O₅
Mol Weight: 272.256
Botanical Source:
Physical Description:
Type of Compound: Flavonoids

Purity: 95%~99%
Analysis Method: HPLC-DAD or/and HPLC-ELSD
Identification Method: Mass, NMR
Packing: Brown vial or HDPE plastic bottle

Storage: Store in a well closed container, protected from air and light. Put into refrigerate or freeze for long term storage.
Whenever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20℃. Generally, these will be useable for up to two weeks.

The product could be supplied from milligrams to grams
Inquire for bulk scale.




Chengdu Biopurify Phytochemicals Ltd. is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，TCM), Natural Active Pharmaceutical Ingredients worldwide.
The main services:
A. Supply active ingredients and reference standards of TCM, from mgs to kgs scale
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR(contract research), CM(contract manufacture) and PD(process development) services from lab scale, pilot scale to commercial scale(GMP is also available)
E. TCM compounds library for drug screen,more than 3000 compounds available, many of them are rare and unique.

Storage conditions：Short-term storage at 2~8℃, long-term storage at -20 ~ -80℃

Introduction/Overview

Butin (CAS No.: 492-14-8) is a natural flavonoid compound originally isolated from the heartwood of Dalbergia spp. As an important member of flavonoid compounds, Zizhenting has attracted widespread attention in recent years due to its remarkable biological activity, especially its antioxidant, anti-platelet aggregation, and anti-inflammatory effects. It shows potential therapeutic value for various diseases, especially pathological processes related to inflammation and tumors. This paper aims to systematically review the chemical structure, origin, pharmacological activity, mechanism of action, and druggability evaluation of Zizhenting, explore its application potential in prostate cancer and other diseases, and look ahead to its future research directions and clinical development prospects.

Chemical structure and physicochemical properties

Zi Ritting is a flavonoid compound with a molecular formula of C15H12O5 and a molecular weight of 272.25. Its structural features include a typical flavonoid backbone containing multiple hydroxyl groups, which imparts excellent antioxidant properties. The LogP value of Zizhenting is 1.5, indicating moderate lipid solubility, which is beneficial for absorption and distribution in the body. Its topological pole surface area (TPSA) is 94.83 Ų, and it has 5 hydrogen bond acceptors. These physicochemical parameters suggest that Zi Ring Pavilion has good bioavailability and cell membrane penetration ability.

Violet riveting has two enantiomers, among which (-)-Violet riveting is the main active form, showing stronger biological activity. This compound has good chemical stability and exhibits low blood-brain barrier penetration in vivo, reducing the risk of central nervous system toxicity. Toxicological evaluation showed that Ziruting had no hepatotoxicity, cardiotoxicity, or hERG channel inhibitory effects, and the Ames-induced mutagenic test was negative, indicating high safety.

Plant Origins and Extraction Methods

Zizhaoting is mainly obtained by extracting the heartwood of the Dalbergia genus. Dalbergia plants are widely distributed in tropical and subtropical regions and are widely used as traditional Chinese medicinal materials. Huangdanxin heartwood is rich in flavonoids, with a relatively high content of Zi Ritting (Zi Rhenting), which is one of the important active components of this genus.

Common methods for extracting Zi Riveting include solvent extraction, column chromatography separation, and high-performance liquid chromatography (HPLC) purification. Generally, ethanol or methanol is used as the extraction solvent, and extraction is performed by reflux or ultrasound-assisted extraction, combined with silica gel column chromatography or C18 reversed phase column for separation and purification. In recent years, the application of supercritical CO2 extraction and membrane separation technologies has improved extraction efficiency and purity, providing technical support for the large-scale preparation of ziriveting pavilions.

Pharmacological activity research

Antioxidant activity

As a polyhydroxyflavonoid, Zizhenting exhibits strong free radical scavenging ability. In vitro experiments show that it can effectively eliminate DPPH and ABTS free radicals, inhibit lipid peroxidation, and protect cells from oxidative damage. Its antioxidant mechanism mainly relies on directly capturing free radicals and regulating intracellular antioxidant enzyme systems (such as SOD, CAT, GPx).

Anti-platelet aggregation effect

Platelet aggregation is an important pathological basis for various cardiovascular diseases. Zizhenting can significantly inhibit platelet activation and aggregation, block calcium flow and related signaling pathways within platelets, and reduce the risk of thrombosis. Both in vitro and animal model experiments have confirmed its good antithrombotic effects, suggesting its potential in preventing and treating thrombotic diseases.

Anti-inflammatory effects

Inflammation is the common pathological basis for many chronic diseases. Zizhenting reduces inflammatory responses by inhibiting the expression of pro-inflammatory factors (such as TNF-α, IL-6, IL-1β). Its mechanism involves regulation of the NF-κB and MAPK signaling pathways, reducing the infiltration of inflammatory cells and the release of inflammatory mediators. In addition, Zizhenting can inhibit the activity of inflammation-related enzymes such as COX-2 and iNOS, exerting multi-target anti-inflammatory effects.

Antitumor activity

Zi Rong Pavilion has shown effects in various tumor cell lines to inhibit proliferation, induce apoptosis, and inhibit migration. Especially in prostate cancer models, Zizhenting inhibits tumor cell growth and invasion by regulating multiple signaling pathways. Its antitumor activity is closely related to its regulatory molecular targets, demonstrating potential as a candidate drug for anti-cancer.

Mechanism of action and molecular targets

The pharmacological effects of Zizhenting involve multiple signaling pathways and key molecular targets, and have been especially elucidated in prostate cancer research. The main targets include:

• BCL2: Zizhenting can downregulate the expression of the anti-apoptotic protein BCL2, promoting tumor cell apoptosis.
• PTPN1: By regulating the protein tyrosine phosphatase PTPN1, Zi Zhan Tian affects cell proliferation and metabolic signaling.
• STAT3: Inhibits STAT3 activation, blocking its tumor-promoting and immune escape effects.
• ESR2: As an estrogen receptor, ESR2 β helps regulate the hormonal response of prostate cancer cells.
• MAPK1: Intervenes in the MAPK signaling pathway, regulating cell proliferation and apoptosis.
• CYP19A1: Affects the expression of aromatase and regulates hormone metabolism.
• AR: Inhibits androgen receptor (AR) signaling, blocking hormone-driven growth of prostate cancer.
• PIK3CA: Regulates the PI3K/AKT pathway, inhibiting tumor cell survival.
• LGALS3: Regulates galactogenin 3, affecting the tumor microenvironment and cell adhesion.
• EGFR: Inhibits epidermal growth factor receptor (EGFR) signaling, reducing tumor cell proliferation and migration.

Ziren Pavilion achieves comprehensive anti-tumor and anti-inflammatory effects through multi-target and multi-pathway synergistic effects. Moreover, its regulation of oxidative stress-related signals further consolidates its potential in disease treatment.

Druggability evaluation and pharmacokinetics

The physicochemical properties of Zi Ring Pavilion indicate that it has promising potential as a finished medicine. Moderate molecular weight and LogP values are beneficial for drug absorption and distribution. Higher TPSA and hydrogen bond receptor counts ensure binding to the target while limiting nonspecific penetration and reducing the risk of toxic side effects.

Toxicological evaluation showed that Zizhaoting showed no significant hepatotoxicity, cardiotoxicity, or genotoxicity, and its safety was good. Its lower blood-brain barrier penetration reduces the likelihood of adverse reactions in the central nervous system.

Pharmacokinetic studies show that Zi Yingting is well absorbed orally, widely distributed in the body, metabolically stable, and mainly excreted through the liver. Moderate half-life, suitable for daily administration. In the future, further in vivo pharmacokinetic and pharmacokinetic studies are needed to optimize dosing regimens and dosage form design.

Prospects and outlooks for clinical applications

As a multifunctional flavonoid natural product, Zizhenting demonstrates broad pharmacological activity and good safety. Its multi-target regulatory effects in prostate cancer, especially its synergistic effects in anti-tumor, anti-inflammation, and antioxidant properties, give it the potential to become a novel anticancer drug.

Future research should focus on:

1. In-depth mechanism analysis: Using multi-omics techniques, further elucidation of the molecular networks and signaling pathways involved in the action of the violet riveting pavilion.
2. Pharmacokinetic optimization: Develop novel delivery systems to improve bioavailability and targeting.
3. Preclinical and clinical research: Conduct systematic toxicological evaluations and clinical trials to verify safety and efficacy.
4. Combination Medication Strategy: Explore the synergistic effects of Ziruting with existing drugs to enhance treatment outcomes and reduce the risk of resistance.
5. Expansion of applications for other diseases: Based on its anti-inflammatory and antioxidant properties, it is exploring applications in cardiovascular diseases, neurodegenerative diseases, and other fields.

In summary, Zizhenting, as a natural flavonoid compound with multiple biological activities, has promising development prospects and is expected to become an important candidate molecule for future natural drug development.

Conclusion

Zizhenting, as the main active flavonoid in Huangtan heartwood, holds an important position in the field of natural product pharmacology due to its excellent antioxidant, antiplatelet aggregation, and anti-inflammatory properties. Its multi-target mechanism of action and good druggability provide a solid foundation for the development of novel anti-tumor and anti-inflammatory drugs. In the future, through systematic pharmacological mechanism research and clinical translation, Zizhengting is expected to become an effective drug for treating prostate cancer and related diseases, injecting new vitality into the development of natural product drugs.