Storage conditions：Short-term storage at 2~8℃, long-term storage at -20 ~ -80℃

Introduction/Overview

Alloimperatorin (CAS No.: 642-05-7) is a natural coumarin-type compound, mainly isolated from the traditional Chinese medicinal material Angelica dahurica. As an important member of the psoralen family, Beo Xianglin has attracted widespread attention in the field of natural product pharmacology in recent years due to its unique structure and significant biological activity. Numerous in vitro and in vivo studies have shown that beeuprofen has good antitumor activity, especially in models of acute myeloid leukemia (AML) cells. In addition, Bieo Qiaofusu also demonstrates regulatory potential for multiple molecular targets related to heart failure, indicating its promising application in cardiovascular diseases.

This paper will systematically review the chemical structure and physicochemical properties of Biaoqianhusu, plant origin, and extraction methods, with a focus on its pharmacological activity and mechanism of action. Combined with druggability evaluation and pharmacokinetic data, it will explore its clinical application potential and future research directions, aiming to provide a theoretical basis and research reference for the drug development of this natural product.

Chemical structure and physicochemical properties

Bieou Qianhu Su belongs to the linear coumarin-class compounds, with the chemical formula C16H14O4 and a molecular weight of 270.28. Its structural core is the psoralen framework, containing a benzene ring and a furan ring, which are connected by oxygen bridges to form a typical coumarin-like structure. The LogP value of Bieo pre-Husu is 3.88, indicating moderate lipid solubility, which is beneficial for cell membrane penetration and bioavailability. Its polar surface area (TPSA) is 55.76 Ų, and it has 4 hydrogen bond acceptors, indicating certain hydrophilicity and binding ability in intermolecular interactions.

In terms of physicochemical properties, bio prehuin exhibits good stability, is easily soluble in organic solvents such as ethanol, methanol, and dichloromethane, and poorly soluble in water. Its molecular structure contains multiple conjugated double bonds and phenolic hydroxyl groups, giving it strong biological activity and photosensitive properties. Notably, Biaoqianhu has a high ability to cross the blood-brain barrier, suggesting its potential application in central nervous system diseases.

Plant Origins and Extraction Methods

Be'ou pre-hull mainly comes from Psoralea (Angelica dahurica), a traditional Chinese medicinal herb widely distributed in East Asia. Psoralea roots are rich in various coumarins, among which the pre-sulfur is relatively abundant. Traditionally, Psoralea has been used to treat rheumatic pain, headaches, and skin diseases, with its medicinal effects closely related to coumarin-based components.

Common methods for extracting bio-prehulin include solvent extraction, ultrasound-assisted extraction, and liquid chromatography separation. Ethanol or methanol is generally used as extraction solvents, and high-purity bieu pre-husu is obtained through multi-step extraction and separation. In recent years, the application of supercritical CO2 extraction technology and high-performance liquid chromatography (HPLC) methods has improved extraction efficiency and purity, reduced impurity interference, and provided technical support for the large-scale preparation of bieu pre-falcon.

Pharmacological activity research

Antitumor activity

Bioprofen exhibits significant antiproliferative effects in various tumor cell lines, especially showing strong cytotoxicity in HL-60 acute myeloid leukemia cells. In vitro experiments have shown that Biaoqianhuin can induce tumor cell apoptosis, disrupt the cell cycle, and inhibit cell migration and invasion capabilities. The mechanism of its antitumor activity involves regulation of multiple signaling pathways, including mitochondrial pathway activation, downregulation of key cell cycle protein expression, and upregulation of pro-apoptotic factors.

In addition, Beoqianhusu also has photosensitive activity, which can enhance the hemolytic effect of red blood cells in photodynamic therapy (PDT), suggesting its potential application value in photosensitization therapy. This characteristic offers new ideas for tumor treatment, especially advantageous in combination therapy strategies.

Cardiovascular protective effects

In addition to its antitumor activity, beta-o-profurin has shown certain protective effects in cardiovascular disease models such as heart failure. By regulating key targets such as AMPK (PRKAA1), EHMT2, APP, PTPN1, and MAOA, Bio Xiang Xiang can improve myocardial energy metabolism, reduce oxidative stress, inhibit myocardial fibrosis and inflammatory responses, thereby slowing the progression of heart failure.

Its regulation of multidrug resistance-related proteins such as ABCB1 and ABCG2 also suggests that biohuin may play a role in improving drug tolerance and enhancing cardiovascular drug efficacy.

Mechanism of action and molecular targets

The pharmacological mechanism of Biaoqianhulin is complex, involving regulation of multiple molecular targets and signaling pathways.

1. Induction of apoptosis: Beoqianhulin promotes cytochrome C release by activating mitochondrial-dependent apoptosis pathways, activating the caspase cascade, ultimately leading to tumor cell apoptosis. It can also regulate the expression of Bcl-2 family proteins, promote upregulation of pro-apoptotic proteins, and inhibit anti-apoptotic proteins.

2. Cell cycle blockade: Beeu prophesine can downregulate the expression of cyclin and cycle-dependent kinase (CDK), blocking cell cycle progression during G0/G1 or G2/M phases and inhibiting tumor cell proliferation.

3. Inhibition of cell migration and invasion: By regulating matrix metalloproteinases (MMPs) and related signaling pathways, biopropanin suppresses tumor cell migration and invasion, reducing the risk of metastasis.

4. Regulates energy metabolism and oxidative stress: Beioqianhuin activates the AMPK signaling pathway, promotes energy metabolism balance, reduces oxidative damage to myocardial cells, and exerts a protective effect on the heart.

5. Multi-target regulation: Beioqianhulin regulates targets such as EHMT2 (a histone methyltransferase), APP (amyloid precursor protein), PTPN1 (protein tyrosine phosphatase 1B), and MAOA (monoamine oxidase A), affecting cell signaling, metabolism, and inflammatory responses.

Druggability evaluation and pharmacokinetics

The druggability evaluation of Biou Qianhusu shows good drug development potential. Its molecular weight is moderate (270.28), with a LogP value of 3.88, meeting the Lipinski rule and indicating good oral bioavailability. TPSA is 55.76 Ų, suitable for cell membrane penetration, and has 4 hydrogen bond receptors, which facilitates binding to target proteins.

Toxicological evaluation indicated that Beoprofen has a low risk of hepatotoxicity, no significant cardiotoxicity, and does not inhibit hERG channels, reducing the likelihood of drug-induced arrhythmias. The Ames test result was negative, indicating no mutagenicity.

In terms of pharmacokinetics, bieo prophesine has a high blood-brain barrier penetration ability, suggesting its potential in treating central nervous system diseases. Its metabolism in the body is stable, with excretion mainly via the liver, and a moderate half-life, making it suitable for further drug formulation development.

Prospects and outlooks for clinical applications

Based on the multiple activities of bioqianhusu in antitumor and cardiovascular protection, its clinical development prospects are broad. Especially in adjuvant therapy for malignant tumors such as acute myeloid leukemia, beoprofen can serve as a potential chemotherapy synergist or photosensitizer for photodynamic therapy. Its ability to regulate multidrug resistance proteins also provides new strategies for overcoming tumor drug resistance.

In the field of cardiovascular diseases such as heart failure, bieuprofen regulates myocardial metabolism and inflammation through multiple targets, and may become a candidate molecule for novel cardioprotective drugs. In the future, further verification of efficacy and safety is needed through animal models and clinical trials.

Additionally, the blood-brain barrier penetration of beoqianhulin suggests its potential application value in neurodegenerative diseases, warranting related research.

Future research should focus on optimizing the drug formulation of beneopronu, clarifying its in vivo metabolic pathways and pharmacodynamic characteristics, conducting systematic toxicological evaluations and preclinical studies, and promoting its clinical application.

Conclusion

Beoqianhusu, a natural coumarin-derived compound derived from psoralea, shows broad application prospects in the fields of anti-tumor and cardiovascular disease treatment due to its unique chemical structure and multi-target pharmacological activity. Its excellent druggability and safety lay a solid foundation for subsequent drug development. In the future, through in-depth mechanistic research and clinical validation, Biou Qianhu Su is expected to become an important candidate molecule in the development of natural product drugs, providing new strategies and approaches for the treatment of related diseases.