| 产品名称 | DYKDDDDK tag Monoclonal antibody |
|---|---|
| 产品编号 | HB40002 |
| Uniprot ID | FLAGTAG |
| Host Species | Mouse |
| Reactivity | recombinant protein |
| Tested Applications | WB, IF/ICC, FC (Intra), IP, ELISA |
| Application | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IF/ICC : 1:200-1:800 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Background | DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology. |
| Alternative Names | Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK |
| Immunogen | Peptide |
| Isotype | IgG2b |
| Conjugate | Unconjugated |
| Purification | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Formulation | Liquid |
| Storage | Store at -20°C |
| Figure ID 2 | HB40002 |
| 曾用货号 | B30003T |
| 保质期 | 12 months |
