| 产品名称 | DYKDDDDK tag Recombinant antibody |
|---|---|
| 产品编号 | HB40001 |
| Uniprot ID | FLAGTAG |
| Host Species | Rabbit |
| Reactivity | recombinant protein |
| Tested Applications | WB, IF/ICC, FC (Intra), IP, ELISA |
| Application | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IF/ICC : 1:125-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Background | Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit recombinant antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology. |
| Alternative Names | 3Flag, Ag2329, Flag, DDDDK, DDDDK tag |
| Immunogen | Recombinant DYKDDDDK tag protein |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Purification | Protein A purfication |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Formulation | Liquid |
| Storage | Store at -20°C |
| Figure ID 2 | HB40001 |
| 曾用货号 | B30002T |
| 保质期 | 12 months |
