| 产品名称 | 6*His, His-tag Monoclonal antibody |
|---|---|
| 产品编号 | HB40000 |
| Uniprot ID | HISTAG |
| Host Species | Mouse |
| Reactivity | recombinant protein |
| Tested Applications | WB, IF/ICC, FC, IP, ELISA |
| Application | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IF/ICC : 1:200-1:800 FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Background | Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein. His-tag is often used for affinity purification and binding assays. Expressed. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. A His-tag (polyhistidine tag) consists of at least six histidine residues that are located at the N- or C-terminus of recombinant proteins. It is commonly used for affinity purification and protein binding experiments. |
| Alternative Names | 6*His, His Tag, His Tag, 6*His, 6*his tag, 6*his,His tag |
| Immunogen | Peptide |
| Calculated MW | 0.84 kDa |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Purification | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Formulation | Liquid |
| Storage | Store at -20°C |
| Figure ID 2 | HB40000 |
| 曾用货号 | B30001T |
| 保质期 | 12 months |
