| 产品名称 | TBP Polyclonal antibody |
|---|---|
| 产品编号 | HB30027 |
| Uniprot ID | P20226 |
| Host Species | Rabbit |
| Reactivity | human, mouse, rat, pig, monkey, sheep |
| Tested Applications | WB, IP, IHC, ELISA |
| Application | WB : 1:500-1:3000 IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC : 1:250-1:1000 |
| Background | The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa. |
| Alternative Names | GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID |
| Immunogen | Recombinant human TBP protein |
| Calculated MW | 338 aa, 38 kDa |
| Observed mw | mouse/rat 33-36 kDa and human 37-43kDa |
| Subcellular Localization | Nuclear |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Purification | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Formulation | Liquid |
| Storage | Store at -20°C |
| Figure ID 2 | HB30027 |
| 保质期 | 12 months |
