| 产品名称 | Beta Actin Recombinant antibody |
|---|---|
| 产品编号 | HB30009 |
| Uniprot ID | P60709 |
| Host Species | Rabbit |
| Reactivity | human, mouse, rat, pig, canine, monkey, chicken, bovine, goat |
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA |
| Application | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC : 1:1000-1:4000 IF/ICC : 1:950-1:3800 |
| Background | Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 ) |
| Alternative Names | ACTB, Actin, bate actin, β actin, 4H1 |
| Immunogen | Recombinant human beta actin protein |
| Calculated MW | 375 aa, 42 kDa |
| Observed mw | 42 kDa |
| Subcellular Localization | Cytoplasm |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Purification | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Formulation | Liquid |
| Storage | Store at -20°C |
| Figure ID 2 | HB30009 |
| 曾用货号 | B31002T |
| 保质期 | 12 months |
