1、试剂盒的运输
    生化试剂盒一般采用冰袋运输；运输中冰袋已融化并不会影响其质量，请放心使用。
2、试剂盒的保存：
    收到后，请在预实验前及时确认各组分量是否与说明书标注的一致，若有疑问请及时与我们技术联系（联系方式见说明书右下方），拆开包装后，请按照每个试剂的保存温度保存。
3、试剂盒有效期
     试剂盒大标签上显示试剂盒有效期，一般是3个月或6个月（具体以大标签为准）。粉体试剂加溶液后的保存周期，可参照说明书中“备注”一栏的说明（也可联系说明书下方的技术支持咨询确认）。
4、预实验五步法
    强烈建议客户选取2-3个预期结果差别较大的样本做预测定。
    目的：了解本批样品情况，熟悉实验流程，避免实验样本和试剂浪费！
    第一步：将试剂盒内的纸质版说明书通读一遍，核查试剂数量、状态（粉体、液体）及各组份的量与说明书是否一致，若无异常则按照规定温度存放。
    第二步：临用前取出试剂，溶解或者恢复至室温，以待使用。
    第三步：选择差异大的2-3个样本进行研磨提取，取上清液备用。
    第四步：根据说明书操作步骤进行预实验。
    第五步：待预实验确定好样本检测浓度、调整情况后，再批量进行正式实验。
5、分光法（F）与微板法（W）的区别
    F:指分光法；W：指微板法;
    分光法是指使用紫外可见分光光度计或可见分光光度计检测，分光法试剂盒(货号中带“F”)，需要使用石英或玻璃比色皿；其规格是：光径：1cm,容积：1mL, 狭缝宽：3mm
    微板法指使用全波段连续酶标仪检测。微板法试剂盒（货号中带“W”），需要使用96孔酶标板或UV板，其规格是：U型底或平底、最大孔容量300μL
6、普通酶标板与UV板的区别？
    使用酶标仪检测时，如果波长大于等于340nm,使用普通的96孔板；但是波长小于340nm时，需要使用UV板（紫外酶标板）；UV板（PS聚苯乙稀石英底）价格比较贵，但是可以清洗，重复使用。一般建议重复使用3-5次；
    最终测定的反应液为有机试剂时（乙酸乙酯、四氢呋喃等有机试剂），避免使用聚苯乙稀材质的96孔板。
7、分光法与微板法试剂盒能混用吗？
    不可以。两者原理几乎一样，但因反应体系、样本用量、试剂浓度、仪器和耗材都不同，并且混用后结果的准确性不能保证，不建议混用与任意改变体系。
8、市面上见到的50T/48S；100T/96S;120T/100S是什么意思？
     T:指测试（Test）次数；在实验室中，这通常指的是反应孔；每个反应孔可以是一个实验样本，或者对照；并不代表测的样本个数。50T是50个反应孔，不一定代表测50个样本； 100T、120T也是如此；
S：指可以测试的样本（Sample）个数；48样、96样：指可以检测48个样本、96个样本（不含重复）即得到48个、96个数据结果
9、官网上试剂盒规格标注的“48样”、“96样”是什么意思呢？
    “48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的；一般会给到超出需用量的10-20%。
    48样、96样：指可以检测48个样本、96个样本（不含重复）即得到48个、96个最终的数据结果。格锐思生物的试剂盒48样可以测不少于50个样本；96样指可以测定不少于100个样本。
10、标准曲线是否需要重新做？
    一般不建议重新做标准曲线，标准曲线绘制会损耗试剂量，不够测到试剂盒规格的样本数量，另外我们的标准曲线是可以溯源，保证标曲的客观、真实、科学严谨，可以直接使用。
    a、格锐思针对需要标曲曲线的指标，给了标准曲线方程，同时给了标准曲线图，以证实我们的标曲是客观做出来的，不是理论推导的。
    b、格锐思同时给了标准曲线绘制的实验的步骤，客户对标曲有疑问的话，可以按照我们步骤，重新做标曲。
    c、格锐思根据标曲推导出最终的计算公式，客户只需要测出吸光值差值与样本重量就可以带入计算。
11、什么是冰浴匀浆？
    （1）使用研钵匀浆：将研钵置于冰盒上匀浆即冰浴匀浆。称取样本置于研钵中，研钵和提取液提前预冷或将研钵置于碎冰、冰盒上，加入一半量的提取液研磨均匀，转移至离心管中，再向研钵中加入另一半量的提取液涮洗研钵，转移至同一离心管中合并；
    （2）使用匀浆机进行匀浆：先把研磨模块提前放到-20℃预冷；然后将称好的组织放入离心管中，加入适量预冷的提取液、钢珠，按照匀浆机要求设定参数，进行研磨，动物植物组织都可以，对于纤维含量高，难磨样本可以提高赫兹和延长时间。或者多次反复研磨
    （3）使用液氮研磨后匀浆：样本液氮研磨成粉后，加入提取液再次按照（1）、（2）的方式研磨，或者放入超声波清洗仪300W超声5min，超声全程温度不得超过10℃。
12、样本的保存
    测常规指标，样本保存在-80℃下，一年以上都可以测；保存在-40℃下，半年之内都可以测；保存在-20℃下，三个月都没问题。

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