荣誉资质介绍
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the known concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. However, the O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), plotting log of the data to establish standard curve for each test is recommended. Typical standard curve below is provided for reference only.
1. Standard Curve
| Standard Concentration（μmol/L） | Assay OD | Absolute OD |
| 0 | 0.0406 | 0 |
| 3.125 | 0.0975 | 0.0569 |
| 6.25 | 0.1581 | 0.1175 |
| 12.5 | 0.2766 | 0.2360 |
| 20 | 0.4117 | 0.3711 |
| 25 | 0.4957 | 0.4551 |
| 50 | 0.9523 | 0.9117 |
Typical Standard Curve for Nitric Oxide Assay
