Mouse Monoclonal Antibodies
Rabbit Polyclonal Antibody Production
A one-stop service from gene design to protein preparation
A one-stop service from gene design to protein preparation
The first step to generate the desired antibodies involves immunizing Balc/c mice with a specific antigen. If you require a custom monoclonal antibody production, our team at ProMab can help you design the peptide sequence for your project. While immunizing mice produces high-affinity and specific antibodies, it is possible for certain antigens and mice to have a stronger immune response than others. To receive a mature immune response, 5 injections of antigen into mice are typically needed; however, it is possible that more or less immunization boosts are required which would increase or decrease the timeline by another 2 to 4 weeks to get the desired titer. Generally, immunization will take approximately 10 weeks.
Following sacrifice, the extracted spleen cells will be fused with compatible myeloma cell lines (Sp 2/0 cells) to generate the hybridoma cells for selection via ELISA. The presence of the desired antibodies is identified using ELISA but additional services including western blot and flow cytometry are available upon request.
The supernatant from 10 hybridoma clones (or more if selected) will be selected based on ELISA performance and sent to the client for in-house evaluation. The client can choose two clones (or more if selected) which we will further subclone and assay to ensure monoclonality and stability. The Custom Mouse Monoclonal Antibody Package starts at $6,995.
Upon confirmation and selection of the desired hybridoma clones, the chosen hybridoma cells will be subcloned to ensure stability and monoclonal characteristic of the cell. The limiting-dilution method will isolate individual hybridomas from the mixed population of cells. The isolated cells will then be expanded and cryopreserved for shipment. If you have any questions about our mouse monoclonal antibody production service, or if you are looking to request a quote, please click below:
| Phase | Week | Description of Process | Included | Additional Cost |
|---|---|---|---|---|
| Phase 0 (Initial Design) | Week 1 | Peptide Design | ✔ | |
| Peptide Synthesis | $750 | |||
| Peptide Conjugation | $300 | |||
| Synthesis + Conjugation | $1000 | |||
| Phase I (Immunization) | Week 2 | Immunization of 5 mice | ✔ | |
| Immunization of 10 mice or more | $300 setup fee $50/mouse | |||
| Week 4 | 2nd Injection of antigen (immunization boost) | ✔ | ||
| Week 6 | 3rd Injection of antigen (immunization boost) | ✔ | ||
| Week 7 | Polyclonal sera collected | ✔ | ||
| Phase | Week 7.5 | ELISA evaluation of titer | ✔ | |
| Phase I | Week 8 Week 9 | 4th Injection of antigen (immunization boost) | ✔ | |
| Polyclonal sera collected | ✔ | |||
| Week 4 | ELISA evaluation of titer | ✔ | ||
| Week 10 | 5th Injection of antigen (immunization boost) | ✔ | ||
| Week 11 | Polyclonal sera collected | ✔ | ||
| ELISA evaluation of titer | ✔ | |||
| Phase II (Cloning) | Week 12 | Picking 10 clones from a pool of 3000 clones | ✔ | $3,595 |
| Picking 11 clones or more | $50/clone | |||
| Negative screening – testing to make sure clones don’t cross-react | $500/antigen | |||
| Screening with Western Blot | ✔ | $500 | ||
| Screening with different applications (IF, FC, IHC) |
✔ | $500/application | ||
| Phase III (Subcloning) | Week 13 | 2 sub-clones will be selected, isotyped and expanded into T25 flasks | ✔ | $1,400 |
| Picking 3 sub-clones or more | ✔ | $500/sub-clone | ||
| Cell line adaptation to serum free media | $1200/sub-clone | |||
| Week 14 | Cryopreservation of hybridoma cells and shipment within the U.S.A. | ✔ |
| Ascites Production ($300 hybridoma setup fee) |
< 11 mice | $60/mouse |
| 11 - 50 mice | $55/mouse | |
| 51 – 99 mice | $50/mouse | |
| >100 mice | $45/mouse | |
| Ascites Purification | Any number of mice | $60/mouse |
| Hybridoma Cells Expansion (Antibody Production) | Hybridoma cells in bioreactor (1 liter of supernatant) | $3,200/clone |
| Hybridoma cells in bioreactor (500ml of supernatant) | $2,600/clone | |
| Antibody Purification (supernatant into 1 mg/ml solution) | 1 liter | $1000 |
| 500 ml | $800 | |
| 100 ml | $500 | |
| Antibody Labeling | FITC | $1000 |
| HRP | $1000 | |
| Biotin | $1000 | |
| Epitope Mapping | Identify the exact epitope of the antigen – antibody binding site | Please inqiure |
| Cold Storage (Liquid Nitrogen) | 2 vials after 6 months | $60/month |
| 3 vials or more | $30/vial/month |
Included in Custom Package
Starting at $2,450
We know how to generate an antibody that is specific to the immunizing antigen and that works for the intended application.
Our technical staff with decades of experience in immunology, biochemistry, and molecular biology will be there to guide your through the 9 to 12-week process.
Immunization and anti-sera production:
Purification:
* It is possible for certain antigens and certain rabbits to have stronger immune response than others. It typically takes 5 injections of antigen into rabbits for a mature immune response. Sometimes additional immunization boosts are required which can add another 2 to 4 weeks to the project. There are also times when it only requires 3 immunization boosts to get the desired titer.
| Service Name | Price |
| *Antigen Preparation-Recombinant protein production | $2,200* |
| Peptide synthesis & conjugation | $750 |
| Extra rabbit | $300 |
| Keep rabbits alive beyond good titer (cost per rabbit per month) |
$100 |
| Peptide or protein affinity purification | $850 |
Each project of recombinant protein customization requires evaluation to determine the corresponding plan and price. For further information on service prices or technical details, please send an email to service@Omnimabs.com Or call +213 / 3753998 to contact us directly.
