| Product Name | Anti-mouse IgG (H+L), F(ab') 2 Fragment (DyLight ® 488 Conjugate) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | This Cell Signaling Technology antibody was conjugated to DyLight® 488 fluorescent dye under optimal conditions and tested in-house for indirect flow cytometric and immunofluorescent analysis of human and mouse cells. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Alligator |
| Tested Applications | FCIF |
| For flow cytometry, use product at a 1:1000 dilution. For immunofluorescence, the optimal dilution may have to be determined. For the detection of many antibodies, the optimal dilution is 1:250-1:1000.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Tissue Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, mouse IgG and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c) or human serum proteins. |
|---|
| Application Notes | For flow cytometry, use product at a 1:1000 dilution. For immunofluorescence, the optimal dilution may have to be determined. For the detection of many antibodies, the optimal dilution is 1:250-1:1000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mg/ml BSA. Store at 4°C. Protect from light. Do not freeze. |
| Storage Buffer | Supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mg/ml BSA. Store at 4°C. Protect from light. Do not freeze. |
Data sheet for OM296310