| Product Name | Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | Anti-Rabbit IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Goat |
| Tested Applications | ChIPFCIFIHCIPWB |
| The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.: |
|
| Species Reactivity | Rabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Tissue Specificity | F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins. |
|---|
Application
Confocal immunofluorescent analysis of P19 cells using LIN28A Antibody detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Application
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, Wortmannin and U0126 (blue), using Phospho-Akt (Ser473) (D9E) Rabbit mAb detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) and compared to a nonspecific negative control antibody (red).| Application Notes | The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM296280