| Product Name | Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | Anti-Mouse IgG (H+L) F(ab')2 Fragment antibody was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Alligator |
| Tested Applications | ChIPFCIFIHCIPWB |
| WB:1:1000~1:5000 |
|
| Species Reactivity | Mouse |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Tissue Specificity | F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum. |
|---|
Application
Confocal immunofluorescent analysis of mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells using SSEA1 (MC480) Mouse mAb detected with anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Application
Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green) compared to a nonspecific negative control antibody (red).| Application Notes | WB:1:1000~1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays. Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM296278